fay-zor
A graphical representation transforming fluorescence decay data into 2D coordinates for fit-free lifetime analysis.
The phasor plot provides intuitive lifetime visualization:
For FRETLoading... analysis, the donor alone phasor and donor+acceptor phasor positions reveal quenching and efficiency.
How to Read It: Points on the curved line = simple single-lifetime decay. Points inside = mixture of lifetimes. Position along the curve tells you the lifetime value.
FRET moves the point toward shorter lifetime (rightward along the curve).
Traditional lifetime analysis requires assuming a decay model (mono-exponential, bi-exponential, etc.) and fitting parameters. Phasor analysis offers advantages:
These properties make phasor analysis particularly suitable for clinical applications requiring standardized, reproducible analysis.
Why Phasor? Traditional analysis requires guessing how many lifetime components exist. Phasor doesn't—it just shows you the data directly. Faster, more reliable, better for clinical use.
