PanoBrain Home PanoBrain Glossary Whole-Slide Brain Scanning
Acquisition

Whole-Slide Brain Scanning

From tissue slide to seamless digital brain section

Definition
Automated acquisition of an entire brain section at high resolution by tiling individual fields of view, stitching them into a seamless mosaic, and producing a single large-format image suitable for atlas registration and quantitative analysis.
Under 1 Minute
Full dual-channel coronal section at 10X
Seamless Mosaic
No visible tile boundaries in the final image

The Scanning Process

A typical mouse brain coronal section spans roughly 10 x 8 mm — far larger than a single microscope field of view. Whole-slide scanning:

  • Intelligent path planning — PanoBrain detects tissue boundaries and plans an efficient scanning path, skipping empty areas
  • Tile acquisition — hundreds of overlapping tiles captured at the selected magnification (10X, 20X, or 40X)
  • Real-time stitching — tiles are seamlessly joined with intensity normalization and sub-pixel alignment
  • Multi-channel — each tile captures all fluorescence channels before moving to the next position

PanoBrain completes a full dual-channel scan in under one minute per section.

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