GFP and its variants are the most widely used fluorescent reporters in neuroscience:
- Viral tracing — AAV vectors express GFP in transduced neurons, revealing injection sites and projection targets
- Transgenic lines — GFP driven by cell-type-specific promoters labels defined neural populations
- Activity reporters — GFP-based calcium indicators (GCaMP) report neural activity
PanoBrain captures GFP signal in its green fluorescence channel alongside DAPI nuclear staining, enabling automated quantification of GFP-positive cells per brain region.