Fluorescence Methods
10
Terms
Term Reference
10 terms| Term | Description | |
|---|---|---|
| Confocal Microscopy | An imaging technique that uses pinholes to reject out-of-focus light, enabling sharp optical sections through thick samp... | |
| Fluorescence | The emission of light by a molecule that has absorbed light at a shorter wavelength. The difference between absorption a... | |
| Fluorophore Fluorophore / Fluorescent Probe | Light-emitting molecules used to label antibodies for FRET detection–the signal source. | |
| Sequential Acquisition | Imaging each fluorescence channel in sequence rather than simultaneously, eliminating spectral bleedthrough at the cost ... | |
| Simultaneous Acquisition | Capturing multiple fluorescence channels in a single exposure using spectral separation and multiple detectors. Eliminat... | |
| smFISH | Single Molecule Fluorescence In Situ Hybridization—a technique for detecting and counting individual mRNA molecul... | |
| Spectral Bleedthrough Spectral Bleedthrough / Crosstalk | Artifact where donor emission contaminates acceptor detection, creating false FRET signal. FLIM-based lifetime measureme... | |
| Tissue Clearing | Chemical treatment to make tissue optically transparent by removing light-scattering components and matching refractive ... | |
| Z-Planes | The number of focal planes captured in a Z-stack, determining the axial extent and resolution of 3D imaging. More planes... | |
| Z-Stack | A series of images captured at different focal planes through the sample, enabling 3D reconstruction, maximum intensity ... |