Fluorescence Methods
10
Terms
Term Reference
10 terms| Term | Description | |
|---|---|---|
| Confocal Microscopy | Optical sectioning through pinhole rejection | |
| Fluorescence | Light in, longer-wavelength light out | |
| Fluorophore Fluorophore / Fluorescent Probe | Light-emitting molecules used to label antibodies for FRET detection–the signal source. | |
| Sequential Acquisition | One channel at a time to avoid bleedthrough | |
| Simultaneous Acquisition | All channels at once | |
| smFISH | Counting individual mRNA molecules | |
| Spectral Bleedthrough Spectral Bleedthrough / Crosstalk | Artifact where donor emission contaminates acceptor detection, creating false FRET signal. FLIM-based lifetime measureme... | |
| Tissue Clearing | Making tissue transparent for deep imaging | |
| Z-Planes | Slices through the sample | |
| Z-Stack | Imaging through the sample volume |