System Performance
11
Terms
Term Reference
11 terms| Term | Description | |
|---|---|---|
| Acquisition Overhead | The time spent during image acquisition that is not collecting photons from the sample. This includes filter wheel chang... | |
| Camera-Objective Matching | The process of selecting camera pixel size and objective magnification to achieve optimal Nyquist sampling while balanci... | |
| Channel Count | The number of distinct fluorescence channels in the imaging experiment. More channels provide more information but incre... | |
| Equivalent Sensitivity | The principle that different camera/objective combinations can achieve similar practical sensitivity through compensatin... | |
| Exposure Time | The duration for which the camera sensor collects photons for each image. Longer exposures collect more signal but slow ... | |
| Photons per Pixel | The number of photons collected by each pixel during an exposure, before conversion to electrons. This is the starting p... | |
| Sample Area per Pixel | The actual area of the sample captured by each camera pixel, determined by pixel size divided by magnification. This met... | |
| Sensitivity Trade-offs | The interplay between optical and detector parameters that determines how efficiently the system converts sample fluores... | |
| Signal-to-Noise Ratio | The ratio of detected signal to the uncertainty (noise) in that measurement. Higher SNR means clearer images and more re... | |
| Throughput | The rate at which sample area is imaged, typically measured in mm²/sec. Throughput integrates exposure time, frame ra... | |
| Total Acquisition Time | The complete time required to acquire all images for a sample, including exposures, readout, mechanical movements, and a... |