Bregma is the origin point of the stereotaxic coordinate system. During surgery, it is visible as the intersection of skull suture lines. During post-hoc analysis, PanoBrain's atlas registration effectively reverse-maps each section back to its Bregma-relative position, enabling:
- Precise AP-level identification of each scanned section
- Comparison of the same brain region across different animals
- Correlation of histological findings with surgical injection coordinates