Acquisition Fundamentals
Scanning, stitching, autofocus, and illumination for whole-brain imaging
5
Terms
Term Reference
5 terms| Term | Description | |
|---|---|---|
| Autofocus for Thick Sections | An adaptive focus strategy that compensates for the uneven topography of thick brain sections by adjusting the Z-positio... | |
| Dual-Channel Acquisition | Simultaneous or sequential capture of two fluorescence channels, typically DAPI for nuclei and GFP for a reporter. The t... | |
| Fluorescence Stitching | The process of joining overlapping fluorescence image tiles into a single large mosaic. Stitching corrects vignetting, i... | |
| Strobe Illumination | A pulsed LED illumination technique that delivers high-intensity light in microsecond bursts synchronized to the camera ... | |
| Whole-Slide Brain Scanning | Automated acquisition of an entire brain section at high resolution, by tiling individual fields of view and stitching t... |