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3D Analysis

Serial Section Analysis

From 2D sections to 3D brain-wide quantification

Definition
The systematic imaging and quantification of sequential brain slices along the anterior-posterior axis. Marker distributions can then be reconstructed in 3D, and labeled cell populations estimated by volume.
Volumetric Estimation
Extrapolate from sampled sections to total volume
Batch Scanning
PanoBrain processes entire series automatically

Spanning the Brain

A typical serial section analysis collects every Nth section (e.g., every 6th at 40 µm) across the relevant AP extent. PanoBrain's batch processing scans all sections automatically, and atlas registration assigns each to its AP level. The result is a dataset spanning millimeters of brain tissue with consistent, automated quantification at every level.

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