A typical mouse brain coronal section spans roughly 10 x 8 mm, far larger than a single microscope field of view. Whole-slide scanning:
- Path planning: PanoBrain detects tissue boundaries and plans an efficient scanning path, skipping empty areas
- Tile acquisition: hundreds of overlapping tiles captured at the selected magnification (10X, 20X, or 40X)
- Real-time stitching: tiles are joined with intensity normalization and sub-pixel alignment
- Multi-channel: each tile captures all fluorescence channels before moving to the next position
PanoBrain is specified to complete a full dual-channel scan in under one minute per section.