| Term | Description | |
|---|---|---|
| Brightfield Imaging | Transmitted-light imaging in which stains absorb light: the standard modality for H&E and IHC slides. | |
| Field of View | One camera frame at one stage position. A whole-slide image is many FOVs stitched into a single composite. | |
| Fluorescence Imaging | Imaging labeled molecules that absorb excitation light and emit longer-wavelength light against a dark field. | |
| Stitching Combining adjacent acquisition tiles into a single composite image | Combines adjacent fields of view into one image, aligning tiles by stage position or by image content. | |
| Virtual Slides | A scanned whole slide stored as a multi-resolution image, viewable from overview down to single cells. |