Camera & Objective Comparison Simulator
Compare microscopy configurations for throughput, sampling, resolution, and field of view. Click the π Learn button to reveal educational tooltips on every element.
System Parameters
π Emission Wavelength
The wavelength affects Airy disk size and resolution limit. Shorter wavelengths (blue) resolve finer details. Set this to match your fluorophore emission peak.
π· Camera Selection
Each camera has a different pixel size and sensor area. Smaller pixels enable finer sampling but collect fewer photons. Larger sensors provide wider field of view.
π¬ Objective Selection
The objective determines magnification, NA (resolution), and working distance. High-NA objectives resolve finer details but have shallower depth of field and shorter working distance.
π³ Binning Mode
2Γ2 binning combines 4 pixels into 1, doubling signal but halving resolution. 4Γ4 combines 16 pixelsβ4Γ signal, ΒΌ resolution. Use when signal is weak.
π Configuration Library
Click configurations to toggle comparison. Compare up to 6 configurations to see trade-offs in resolution, throughput, and field of view. Use β to edit, β§ to duplicate, Γ to remove.