Camera & Objective Comparison Simulator
Compare microscopy configurations for throughput, sampling, resolution, and field of view. Click the 📖 Learn button to reveal educational tooltips on every element.
System Parameters
🌈 Emission Wavelength
The wavelength affects Airy disk size and resolution limit. Shorter wavelengths (blue) resolve finer details. Set this to match your fluorophore emission peak.
📷 Camera Selection
Each camera has a different pixel size and sensor area. Smaller pixels enable finer sampling but collect fewer photons. Larger sensors provide wider field of view.
🔬 Objective Selection
The objective determines magnification, NA (resolution), and working distance. High-NA objectives resolve finer details but have shallower depth of field and shorter working distance.
🔳 Binning Mode
2×2 binning combines 4 pixels into 1, doubling signal but halving resolution. 4×4 combines 16 pixels—4× signal, ¼ resolution. Use when signal is weak.
📋 Configuration Library
Click configurations to toggle comparison. Compare up to 6 configurations to see trade-offs in resolution, throughput, and field of view. Use ✎ to edit, ⧉ to duplicate, × to remove.