S.NEST from CYTENA BPS is a next-generation incubator-integrated platform that enables continuous, long-term monitoring of live cells under controlled conditions. It supports multi-parametric analysis, allows precise perturbations at defined time points, and preserves cells for downstream experiments.
Conventional assays of mitochondrial function provide only static snapshots of dynamic processes, often requiring repeated experiments to estimate drug response timing and metabolic adaptation. By reducing repetition and consumable use, SNEST offers a time and resource-efficient approach that delivers deeper and more comprehensive insights into cellular bioenergetics.
S.NEST Key Features
- Homogeneous low shear mixing to prevent hypoxia and ensure natural and reproducible growth conditions
- Continuous, and regular readout of dissolved oxygen and pH (DO and ECAR) across all wells
- Non-invasive optical sensors preserve culture integrity and do not interfere with your culture
- Parallel experiments in 24 or 96 wells with independent chamber control
- Detection of growth phases, viability changes, and metabolic shifts without sampling
Fully adjustable low shear mixing ensures uniform oxygenation within each well. Integrated colorimetric sensors at the base of every well track dissolved oxygen and pH across all cultures. S.NEST provides uninterrupted data collection every five minutes without disturbing your culture.
S.NEST’s non-invasive design preserves viability while capturing critical information on metabolic activity, growth phases, and shifts in cellular behavior. By combining precise environmental control with longitudinal assays, the S.NEST gives researchers a dynamic longitudinal window into how cells respond to conditions over hours, days, or weeks.
Four independently controlled incubation chambers allow parallel experiments in 24- or 96-well formats, supporting up to 384 cultures at once. Homogenous mixing minimizes shear while ensuring consistent oxygen transfer, making it possible to run long-term proliferation studies, extended drug exposure assays, or fed-batch optimization without sample destruction. Researchers can detect exponential growth, stationary transitions, viability changes, and metabolic shifts in real time, identifying optimal feeding or harvest points with confidence.
Scalable and versatile, the S.NEST streamlines cell line development and 3D culture monitoring, and also integrates with other ScientiaLux partner technologies, linking metabolic profiles to imaging, single-cell analysis, and high-resolution respirometry for a deeper, systems-level understanding of biology.