The Problem

Current Biomarker Testing
Isn't Good Enough

Immunotherapy has transformed cancer treatment. But we're still using biomarkers that fail to predict who will actually benefit—leaving patients and physicians guessing.

70-80%
of patients don't respond to checkpoint inhibitors
42%
of patients miscategorized by PD-L1 expression testing
$150K+
annual cost per patient for immunotherapy

The Promise and the Problem

Checkpoint inhibitors like pembrolizumab and nivolumab have revolutionized cancer treatment. For some patients, these drugs produce responses that last years—turning a terminal diagnosis into a manageable condition.

But here's the challenge: only 20-30% of patients actually respond. The rest experience the side effects—sometimes severe—without the benefit. And our current testing methods can't reliably tell us who will be in which group.

📊

Expression ≠ Function

Current PD-L1 tests measure how much protein is present. But a protein can be present without being active. It can be in the wrong location. It might not be engaged with its binding partner.

🎯

Missing Responders

24% of patients who could benefit from immunotherapy have low PD-L1 expression. They're denied treatment based on a test that measures the wrong thing.

💊

Treating Non-Responders

18% of patients with high PD-L1 expression don't respond to treatment. They endure toxicity without benefit because the test said they should respond.

🔬

No Mechanism Insight

Expression testing tells you a protein exists. It doesn't tell you if the immune evasion mechanism is actually active—the very thing the drug is designed to block.

"If a drug works by blocking an interaction, we should measure whether that interaction is happening—not just count molecules."

Two Approaches, Different Answers

Current Standard
Expression Testing (IHC)
"How much PD-L1 is present?"
P = 0.162
Failed to predict survival in NSCLC
vs
Functional Approach
Engagement Testing (iFRET)
"Is PD-1 actually binding PD-L1?"
P < 0.0001
Strongly predicted survival

There's a Better Way

What if we measured what actually matters—whether immune checkpoint proteins are actively engaged? Not just present, but functioning. Not just expressed, but interacting.

That's exactly what functional biomarkers do. By measuring protein-protein interaction at nanometer resolution, we can see whether the immune evasion mechanism is truly active—and predict whether blocking it will help.

survival difference detected
(31 vs 10 months)
4.4×
stratification with dual checkpoint profiling
FFPE
works with standard pathology samples

Ready to Learn More?

Discover how functional biomarkers can transform patient selection and improve outcomes.