Fluorescence stitching is more demanding than brightfield because:
- Vignetting — illumination intensity falls off at tile edges, creating visible seams if uncorrected
- Photobleaching — overlapping regions may have reduced signal from double exposure
- Background variation — autofluorescence varies across the tissue, complicating intensity normalization
PanoBrain's stitching engine corrects all of these artifacts, producing images where cell detection algorithms see no tile boundaries.