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Coordinate System

Stereotaxic Coordinates

Precise 3D brain targeting from skull landmarks

Definition
A three-dimensional coordinate system referenced to skull landmarks that enables precise, reproducible targeting of brain structures for injection, implantation, or post-hoc section matching.
Bregma-Referenced
AP and ML measured from the Bregma skull landmark
Reproducible Targeting
Same coordinates hit the same brain region across animals

The Three Axes

Stereotaxic coordinates define positions in the brain along three axes:

  • Anterior-Posterior (AP) — measured from Bregma, positive values anterior (toward the nose), negative values posterior (toward the cerebellum)
  • Medial-Lateral (ML) — measured from the midline, always positive, increasing toward the ears
  • Dorsal-Ventral (DV) — measured from the brain surface or skull, increasing in depth

A coordinate like AP -2.0, ML 1.5, DV 1.2 precisely identifies a point in the hippocampus.

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